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Image Search Results
Journal: Microbiology Spectrum
Article Title: Attenuation of Avian Flavivirus by Rewiring the Leucine and Serine Codons of Its E-NS1 Protein toward Stop Mutation To Redirect Virus Evolution
doi: 10.1128/spectrum.02921-22
Figure Lengend Snippet: Immunogenicity characteristics of CQW1-OTS in 25-day-old ducks. (A) Survival rate of duck postinfection. The statistical significance of survival was analyzed using a survival curve and the log-rank (Mantel-Cox) test, with significance defined. Ns, not significant. n = 5. (B) Viral copies (at 3 and 5 dpi) in the heart, liver, spleen, lung, kidney, brain, and thymus of 25-day-old ducks were detected by RT-qPCR. (C) Relative mRNA expression of IFN-α, IFN-β, and IL-1β in duck spleen at 5 dpi. (D) Peripheral T-lymphocyte (from infected ducks) proliferation activated by TMUV infection. (E) Serum (14 dpi) levels of IFN-γ and IL-4 were determined using ELISA. (F) Neutralization antibody levels in the serum were determined with PRNT. Statistical significance was determined using an unpaired t test. Ns, not significant; *, P < 0.05; **, P < 0.01; ****, P < 0.0001.
Article Snippet: Lymphocytes were separated from blood using a
Techniques: Immunopeptidomics, Quantitative RT-PCR, Expressing, Infection, Enzyme-linked Immunosorbent Assay, Neutralization